胚胎時差培養箱Embryoscope基于其強大的決策支持工具KID Score D3和KID Score D5模型，通過胚胎的形態學及形態動力學，對胚胎進行篩選評分，選擇優質的胚胎進行冷凍或者移植。胚胎形態學包括多核化，細胞碎片化，細胞分裂均勻度等，其中囊胚的形態學是KID Score D5模型中的重要指標，主要通過評估囊胚的擴張程度（發育速度），卵裂形態和碎片等情況可初步篩選出相對優質的囊胚，下文即為基于囊胚的形態學（擴張程度）與其整倍性，妊娠率等的相關性研究：
Blastocyst contractions are strongly related with aneuploidy, lower implantation rates and slower embryo cleavage timing: time lapse study
E Gazzo1, F Pe?a1, F Valdéz2, A Chung1, M Velit1, M Ascenzo1, E Escudero1
1Clínica de Fertilidad INMATER, Lima, Peru. 2Genomics Peru, Lima, Peru.
Objectives: We set out to identify contraction (CT) patterns in human blastocyst and their correlation with their ploidy status (PGT-A analysis), the time they took to reach blastocyst and the pregnancy rate.
Methods: Design: Retrospective study. 912 embryos from 270 patients that went through our center were evaluated. All embryos were cultivated in the Embryoscope incubator. From 912 embryos that started the cohort, 778 were tested for aneuploidy using an NGS platform in a reference laboratory. Blastocyst contractions (CT) were evaluated using the "embryo drawing tool" to obtain the CT percentage. 182 SET were performed, and the mean patient age was 30.44 years, ranging from 24 to 39 years.
Results: Embryos was divided in two groups, those that contracted (CT) and those that did not, we call them "solo expanding" (SE). When this was compared to ploidy status the embryos that SE showed 58.33% to be euploid while 53.58% of embryos that CT where aneuploid. This was statistically significant (p=0.029) showing that embryos that don't contract SE have higher chances of being euploid that embryos that contract CT. When we compared pregnancy rates, we also saw an increase in embryos that SE (63.10%) with embryos that CT (46.67%), this was also statistically significant (p=0.012). Finally, we saw that embryos that CT take longer to reach blastocyst stage compared to SE embryos. This also was significant (p=0.004). Finally we evaluated if the patients age an impact on whether the embryo was CT or SE and found no difference, showing that age should not be a factor y embryo contraction.
Conclusion: The results in this study show statistically significance in two analyzed traits. We showed that CT embryos show a higher chance of being aneuploid, and that they have a lower implantation rate. Also, we showed that embryos that CT take longer to reach blastocyst stage. All this was showed to happen regardless of maternal age. This proves that looking for embryo contractions could be helpful for selecting an embryo for transfer.
摘要：為了研究分析胚胎的皺縮（CT）狀態及其與染色體倍性狀態（PGT-A）、達到囊胚所需的時間和妊娠率相關性?；仡櫺匝芯?，評估了來自生殖中心的270名患者的912個胚胎。所有胚胎均在Embryoscope時差培養箱中培養。在該研究組的912個胚胎中，有778個胚胎使用NGS技術進行非整倍性檢測。使用“胚胎繪圖工具”評估囊胚皺縮（CT），以獲得胚胎皺縮百分比。評估了182組樣本，患者平均年齡為30.44歲，患者年齡在24至39歲之間。將胚胎分為兩組，皺縮組(CT)和未皺縮組(SE，自由擴張組)。將其與倍性狀態進行比較，發現SE組胚胎中為整倍體的占58.33%，而CT組胚胎為非整倍體的占53.58%，p=0.029，兩者有明顯的的差異，表明未皺縮組（SE）胚胎比皺縮（CT）組胚胎有更高的機會成為整倍體。當比較兩組的妊娠率時，也發現SE組胚胎(63.10%)相對CT組胚胎(46.67%)有較大的增長，p=0.012兩者具有明顯的差異。同時，相對于SE組胚胎，CT組胚胎需要更長的時間才能到達囊胚期，p=0.004也具有顯著的差異。最后，評估了患者的年齡對胚胎皺縮或者自由擴張的影響差異，但沒有發現差異，表明年齡不是影響胚胎皺縮的因素。本研究的結果在兩個分析的性狀上具有統計學意義，發現CT胚胎顯示出非整倍體的可能性更高，并且它們的妊娠率較低。此外，顯示CT組胚胎到達囊胚期所需的時間更長。實驗結果表明，這一切未顯示與孕婦年齡相關。因此觀察胚胎皺縮可能有助于選擇需要移植的胚胎。